Device

Part:BBa_M36800:Design

Designed by: Richard Li   Group: Stanford BIOE44 - S11   (2014-04-30)


Acetaldehyde Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A weaker promoter was used for the downstream actuator in case the AlcR had an activating effect on the AlcR operon.


Source

The AlcR sequence was generated from Talaromyces stipitatus and codon-optimized for E. coli.

References