Device
Part:BBa_M36800:Design
Designed by: Richard Li Group: Stanford BIOE44 - S11 (2014-04-30)
Acetaldehyde Sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A weaker promoter was used for the downstream actuator in case the AlcR had an activating effect on the AlcR operon.
Source
The AlcR sequence was generated from Talaromyces stipitatus and codon-optimized for E. coli.